Procedure
- Fill two capillary tubes approximately 2/3 to 3/4 full with the well-mixed blood sample.
- Seal the dry end of the capillary tube by placing it into the sealing clay at a 90o angle.
- Place the capillary tubes in the microhematocrit centrifuge with the sealed end toward the periphery. …
- Centrifuge for five minutes.
Hereof, how do you calculate hematocrit?
Using either a hematocrit reader or any ruled apparatus, measure the length of the column of the packed red cells and divide it by the length of the whole column of blood (cells and plasma), as in Figure 151.1. To obtain the hematocrit, multiply this number by 100%.
In respect to this, how do you read a Microhematocrit tube reader?
How do you use a Hematastat?
Three simple steps
- Fill up to six tubes half to three quarters full.
- Seal the sample tube, place it into the centrifuge and spin for 60 seconds.
- Read tubes on built-in reader tray. Results are displayed on screen.
How does a Microhematocrit tube work?
Thin capillary tubes of various material compositions used for collecting blood samples via capillary action and measuring the volume percentage of red blood cells in those samples; tubes may be heparinized to prevent blood clotting.
What are Microhematocrit tubes?
Also called Microhematocrit Tubes, taken from the Merriam Webster Dictionary definition, the word “Microhematocrit” means: “A procedure for determining the ratio of the volume of packed red blood cells to the volume of whole blood by centrifuging a minute quantity of blood in a capillary tube coated with heparin.”
What does a spun Microhematocrit tube determine?
Microhematocrit. The reference method recommended by NCCLS of determining hematocrit or packed cell volume (PCV) is centrifugation. Method [4]: Hematocrit (PCV) is the measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood.
What does Microhematocrit measure?
Medical Definition of microhematocrit
1 : a procedure for determining the ratio of the volume of packed red blood cells to the volume of whole blood by centrifuging a minute quantity of blood in a capillary tube coated with heparin.
What is high speed centrifuge?
Definition: A separation method where components of a sample are separated on the basis of their density in a centrifuge according to the centrifugal force they experience. Samples are spun at >5000 rpm.
What is PCV in human body?
The packed cell volume (PCV) is a measurement of the proportion of blood that is made up of cells. The value is expressed as a percentage or fraction of cells in blood. For example, a PCV of 40% means that there are 40 millilitres of cells in 100 millilitres of blood.
What is the difference between a Microhematocrit and a regular hematocrit?
Microhematocrit method is a gold standard method for hematocrit determination but it associates many problems that may lead to inaccurate and imprecise measurements. Spun hematocrit is 1% to 3% higher than the hematocrit from automated instrument due to plasma that is trapped in erythrocytes.
What is the main function of Microhematocrit centrifuge?
Microhematocrit centrifuges are used for determination of volume fractions of erythrocytes (red blood cells) in blood and for separation of micro volumes of blood and solutions. It provides hematocrit values (HCT) for testing in biochemistry, immunity, genetics, blood separation and for general clinical tests.
What is the procedure to perform Microhematocrit?
For the microhematocrit method, after filling the blood in a capillary tube, the two ends of the tube (commonly 75 mm long, 1 mm diameter) are sealed with clay sealant or heat. Then, it is centrifuged at a rate of 11000 to 12000 rpm for 4 to 5 min.
What samples are placed in a Microhematocrit centrifuge?
Question: In a microhematocrit centrifuge, small samples of blood are placed in capillary tubes with heparin, an anticoagulant. The tubes are rotated at 11,500 rpm, with the bottom of the tube being 9.07 cm from the axis of rotation.