Take Care of Your Pipette
- Have Your Pipette Serviced Every 6–12 Months. …
- Check Your Pipette for Damage Daily. …
- Clean Your Pipette Each Day Before Use. …
- Store Your Pipette Vertically, Using a Pipette Holder. …
- Never Put Your Pipette on Its Side With Liquid in the Tip. …
- Use Well-Fitting Tips.
In respect to this, how do you handle a pipette?
Put the pipette—held at 10–45 degrees—against the wall of the receiving chamber, and smoothly depress the plunger to the first stop. Wait one second and then depress the plunger to the second stop. Slide the tip up the vessel wall to remove the pipette. Allow the plunger to return to its rest position.
- Temperature. Temperature has many effects on pipetting accuracy. …
- Density. The density (mass/volume ratio) affects the liquid volume that is aspirated into the tip. …
- Altitude. Geographic altitude affects accuracy through air pressure.
Herein, what are the five common pipetting errors?
Common pipetting errors include:
- Failure to Pre-Wet Pipette Tip. …
- Disregarding Temperature. …
- Tip Wiping. …
- Choosing the Wrong Pipetting Mode. …
- Working Too Quickly. …
- Pipetting at an Angle. …
- Using the Wrong Pipette Tips.
What are the hazards of mouth pipetting?
The most common hazards associated with pipetting procedures are the result of mouth suction. Oral aspiration and ingestion of hazards associated with pipetting procedures are the result of mouth suction.
What ergonomics risk are related to pipetting?
Potential problems. Manual action pipettes, one of the most commonly used laboratory instruments, can cause muscle strain or tendon swelling, particularly if good body mechanics are not applied while pipetting. Manual pipetting involves several ergonomic stresses of the wrist, arm, and shoulders.
What is a pipette pump?
Pipette Pumps
… dispenser pipette pumps provide safe and accurate pipetting and dispensing of liquids from serological pipettes. Glass or plastic pipettes are inserted into the tapered collar and the thumbwheel is rotated for precision filling or dispensing.
What is mouth pipette?
Mouth pipetting is the practice of using one’s mouth to suck a desired volume of a medical laboratory specimen–blood, urine, cell cultures and other microbial stews–into an open-ended tube, using the reduced air pressure created by sucking to hold the specimen in place while moving it to another vessel.
What is the error of a pipette?
The systematic error is the deviation of the mean volume measured for a pipette from the volume set on the pipette counter. The systematic error specification is a fixed range outside of which measured mean volumes are not allowed.
What is the purpose of pipette?
About Pipettes. A pipette is a laboratory instrument used to measure out or transfer small quantities of liquid, in volumes of milliliters (mL), microliters (μL).
Why should you not pipette by mouth?
Do not ever use your mouth to pull the liquid into a pipet. This is the most common method of becoming poisoned in a chemical laboratory or becoming infected in a clinical laboratory. Also, having the bulb attached to the pipet increases the risk of drawing the solution into the bulb. …